ATCC medium: 80 Lactic bacteria broth
Trypticase Peptone (BD 211921)..........10.0 g
Yeast autolysate.........................5.0 g
Sodium acetate..........................12.0 g
Glucose.................................10.0 g
Solution A (see below)...................5.0 ml
Solution B (see below)...................5.0 ml
Distilled water to.......................1.0 L
Adjust pH to 5.1-5.3.
Autoclave at 121C for 15 minutes.
Solution A: K2HPO4 ..................................10.0 %
KH2PO4 ..................................10.0 %
Solution B: MgSO4 ....................................4.0 %
NaCO.....................................0.2 %
FeSO4 ....................................0.2 %
MnSO4 ....................................0.2 %