ATCC medium: 2122 YMA medium
Mannitol....................10.0 g
KH2PO4 .......................0.5 g
Sodium glutamate.............0.5 g
NaCl........................50.0 mg
Solution A (see below)......10.0 ml
Solution B (see below).......1.0 ml
Solution C (see below).......1.0 ml
Yeast extract................1.0 g
Agar........................20.0 g
Distilled water to...........1.0 L
Heat to boiling; autoclave at 121C for 15 minutes.
Solution A:
MgSO4 . 7H2O .................1.0 g
Distilled water............100.0 ml
Solution B:
CaCl2 . 2H2O .................5.28 g
Distilled water............100.0 ml
Solution C:
FeCl3 . 6H2O .................0.66 g
Distilled water............100.0 ml