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ATCC medium: 1655 BAM

中國微生物菌種查詢網(wǎng) / 2017-01-06 10:28:54

ATCC medium: 1655 BAM

CaCl2 . 2H2O .......................0.25 g

MgSO4 . 7H2O .......................0.5 g

(NH4)2SO4...........................0.2 g

KH2PO4 .............................3.0 g

Yeast extract......................1.0 g

Glucose............................5.0 g

Trace Elements (see below).........1.0 ml

Agar (if needed)..................30.0 g

Distilled water....................1.0 L

Adjust medium for final pH 4.0 with H2SO4. Autoclave agar separately to avoid acid hydrolysis. Autoclave medium at 121C for 15 minutes.

Trace Elements:

CaCl2 . 2H2O .......................0.66 g

ZnSO4 . 7H2O .......................0.18 g

CuSO4 . 5H2O .......................0.16 g

MnSO4 . 4H2O .......................0.15 g

CoCl2 . 6H2O .......................0.18 g

H3BO3 ..............................0.10 g

Na2MoO4 . 2H2O......................0.30 g

Distilled water....................1.0 L

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