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ATCC medium: 1590 Natronobacteria medium

中國(guó)微生物菌種查詢網(wǎng) / 2017-01-06 12:43:35

ATCC medium: 1590 Natronobacteria medium

KH2PO4 .........................................1.0 g

KCl............................................1.0 g

NH4Cl ..........................................1.0 g

MgSO4 . 7H2O ...................................0.24 g

CaSO4 . 2H2O ...................................0.17 g

Trace Elements Solution SL-6 (see below).......1.0 ml

NaCl.........................................200.0 g

Sodium glutamate...............................1.0 g

Yeast extract..................................5.0 g

Casamino acids.................................5.0 g

Na2CO3 (sterilized as a separate solution) .....5.0 g

Agar..........................................20.0 g

Distilled water to.............................1.0 L

Autoclave basal medium at 121C for 15 minutes. Add Na2CO3 solution. Adjust medium, if necessary, to a final pH of 9.0.

Trace Elements Solution SL-6:

ZnSO4 . 7H2O .................0.10 g

MnCl2 . 4H2O .................0.03 g

H3BO3 ........................0.3 g

CoCl2 . 6H2O .................0.2 g

CuCl2 . 2H2O .................0.01 g

NiCl2 . 6H2O .................0.02 g

Na2MoO4 .H2O..................0.03 g

Distilled water..............1.0 L

Adjust final pH of Trace Elements Solution SL-6 to 3.4.

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