ATCC medium: 2103 OM2 medium
Ammonium oxalate............15.0 g
NaHCO3 ......................10.0 g
NaH2PO4 . 2H2O...............10.0 g
NaCl.........................0.7 g
KCl..........................0.57 g
MgCl2 . 6H2O .................0.1 g
CaCl2 . 2H2O .................0.01 g
Na2S2O3 . 5H2O ................1.0 g
Deionized water..............1.0 L
Dissolve first three salts in the indicated sequence. Add 1.5 to 2.0% Bacto-Agar for solid medium.
Boil medium to dissolve agar. Adjust pH to 6.8-7.0, then autoclave at 121C for 15 minutes.
The pH will shift after autoclaving to 8.5-9.0.