ATCC medium: 1203 CM4 medium
KH2PO4 .............................1.5 g
K2HPO4 .............................2.9 g
Sodium thioglycollate..............0.5 g
Resazurin (1.0% solution)..........0.2 ml
(NH4)2SO4...........................1.3 g
MgCl2 ..............................0.75 g
NaCl...............................1.0 g
CaCl2 .............................13.2 mg
FeSO4 (1.25% solution) .............0.1 ml
Carbon source (i.e. cellobiose)....6.0 g
Yeast extract......................5.0 g
Distilled water....................1.0 L
Agar (if needed)..................20.0 g
Mix all of the ingredients and boil until reduced, indicated by a change from red to colorless.
Allow to partially cool and tube under 97% N2, 3% H2 and cap with butyl rubber stoppers. Autoclave at 121C for 15 minutes.