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ATCC medium: 2062 Desulfitobacterium frappieri medium

中國百歐生物 / 2017-01-09 12:34:45

ATCC medium: 2062 Desulfitobacterium frappieri medium

 KH2PO4 .............................0.27 g

K2HPO4 .............................0.35 g

NH4Cl ..............................0.53 g

MgCl2 . 6H2O .......................0.10 g

FeCl2 . 4H2O .......................2.0 mg

CaCl2 . 2H2O ......................73.0 mg

SL-6 Trace Elements (see below)....1.0 ml

Resazurin..........................1.0 mg

Yeast extract......................1.0 g

Sodium sulfite.....................0.63 g

Sodium pyruvate....................6.05 g

Na2S . 9H2O ........................0.05 g

Distilled water....................1.0 L

Adjust medium, if necessary, for final pH 7.5. Prepare and dispense medium anaerobically under an atmosphere of 80% N2, 10% CO2, and 10% H2.

Autoclave at 121C for 15 minutes.

SL-6 Trace Elements:

ZnSO4 . 7H2O .................0.10 g

MnCl2 . 4H2O .................0.03 g

H3BO3 ........................0.3 g

CoCl2 . 6H2O .................0.2 g

CuCl2 . 2H2O .................0.01 g

 NiCl2 . 6H2O .................0.02 g

Na2MoO4 . 2H2O................0.03 g

Distilled water..............1.0 L

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