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ATCC medium: 1625 Super MMB

中國百歐生物 / 2017-01-09 12:55:34

ATCC medium: 1625 Super MMB

Peptone........................................0.40 g

Yeast extract..................................1.00 g

NH4Cl ..........................................0.20 g

CaCl2 . 2H2O ...................................0.01 g

NaCl...........................................0.20 g Mg

SO4 . 7H2O ...................................0.20 g

Ferric citrate.................................5.0 mg

Trace Elements Solution SL-6 (see below).......1.0 ml

Vitamin Solution (see below)..................20.0 ml

KH2PO4 .........................................1.0 g

Sodium succinate...............................0.4 g

Distilled water................................1.0 L

Adjust medium for final pH 7.0. Autoclave at 121C for 15 minutes.

Trace Elements Solution SL-6:

ZnSO4 . 7H2O .......................0.10 g

MnCl2 . 4H2O .......................0.03 g

H3BO3 ..............................0.3 g

CoCl2 . 6H2O .......................0.2 g

CuCl2 . 2H2O .......................0.01 g

NiCl2 . 6H2O .......................0.02 g

Na2MoO4 . 2H2O......................0.03 g

Distilled water....................1.0 L

Adjust final pH of Trace Elements Solution SL-6 to 3.4.

Vitamin Solution:

Biotin.............................2.0 mg

 Folic acid.........................2.0 mg

Thiamine . HCl.....................5.0 mg

Calcium D-(+)-pantothenate.........5.0 mg

Vitamin B12........................0.1 mg

Riboflavin.........................5.0 mg

Distilled water....................1.0 L

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