ATCC Medium: 2 Marine Agar/Broth 2216
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ATCC Medium: 2 Marine Agar/Broth 2216
Broth Medium
Marine Broth 2216 (BD 279110)……………37.4 g
DI Water…………………………….…….…...1000 ml
Dispense as required (make sure medium is spinning continuously during dispensing). Autoclave at 121?C.
To make solid medium, substitute Marine Broth 2216 with 55.1 grams of Marine Agar 2216 (BD 212185).
To make medium from scratch, follow formulation below:
Difco Marine Agar 2216 Composition
Peptone……………………….….…5.0 g
Yeast Extract………………………..1.0 g
Ferric Citrate……………………..…0.1 g
Sodium Chloride……………….….19.45 g
Magnesium Chloride………...……8.8 g
Sodium Sulfate………………….…3.24 g
Calcium Chloride……………….…1.8 g
Potassium Chloride……………….0.55 g
Sodium Bicarbonate………………0.16 g
Potassium Bromide…………....….0.08 g
Strontium Chloride………….…….0.034 g
Boric Acid……………………..…....0.022 g
Sodium Silicate………………..…..0.004 g
Sodium Fluoride…………….…….0.0024 g
Ammonium Nitrate…………...……0.0016 g
Disodium Phosphate………….…..0.008 g
Agar…………………………………15 g
Distilled water………………...……1000 ml
*Omit agar for broth medium
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