ATCC Medium: 2836 BHI Agar/Broth w/ 100 mcg/ml Rifampicin
Agar Medium
Brain Heart Infusion Agar (BD 211065 )……………52 g
Rifampicin Solution (see below, add aseptically)….50 ml
DI Water………………………………………………...950 ml
Autoclave at 121?C. Cool sterilized medium to ~50?C. Aseptically add 50 ml of
filter sterilized rifampicin solution. Gently mix and dispense as required.
Broth Medium
Brain Heart Infusion (BD 237500 )…………….……37.0 g
Rifampicin Solution (see below, add aseptically)….50 ml
DI Water………………………………………………...950 ml
Autoclave at 121?C. Cool sterilized medium to ~50?C. Aseptically add 50 ml of
filter sterilized rifampicin solution. Gently mix and dispense as required.
Rifampicin Solution
Rifampicin………………………………………………0.1 g
DI Water…………………………………………………50 ml
Filter sterilize and add to sterile base.