ATCC medium: 431 Trypanosome medium
Solid Phase:
Beef extract.................3.0 g
Peptone......................5.0 g
NaCl.........................8.0 g
Agar........................15.0 g
Distilled water..............1.0 L
Liquid Phase (Locke's solution):
NaCl.........................8.0 g
KCl..........................0.2 g
CaCl2 ........................0.2 g
KH2PO4 .......................0.3 g
Glucose......................2.5 g
Distilled water..............1.0 L
Adjust pH of both phases to 7.2-7.4. Autoclave both phases at 121C for
15 minutes. Cool the solid phase mixture to about 45C and aseptically
add 30% sterile, defibrinated, rabbit blood. Aseptically dispense in
sterile 16 X 125 mm screw-capped test tubes in 5 ml amounts. Slant.
After cooling the slants, aseptically dispense 3.0 ml of Locke's
solution (Liquid phase) over each slant.
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