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ATCC medium: 1567 Pedomicrobium PSM
Yeast Extract (BD 212750)................0.5 g
Trace Elements Solution (see below)......1.00 ml
Distilled water........................979.00 ml
Adjust medium for a final pH of 7.1 with KOH. Autoclave at 121C for 15
minutes. Cool and aseptically add:
Sodium acetate..........................10.0 mM
Vitamin Solution (see below)............10.0 ml
Trace Elements Solution:
EDTA...................................125.00 mg
ZnSO4 . 7H2O ...........................547.50 mg
FeSO4 . 7H2O ...........................250.00 mg
MnSO4 . H2O .............................77.00 mg
CuSO4 . 5H2O ............................19.60 mg
Co(NO3)2 . 6H2O..........................12.40 mg
Na2B4O7 . 10H2O ...........................8.85 mg
Distilled water.........................50.00 ml
Vitamin Solution:
D-(+)-Biotin.............................2.00 mg
Folic acid...............................2.00 mg
Thiamine . HCl...........................5.00 mg
Calcium D-(+)-pantothenate...............5.00 mg
Cyanocobalamine..........................0.10 mg
Riboflavin...............................5.00 mg
Nicotinic acid...........................5.00 mg
p-Aminobenzoic acid......................5.00 mg
Pyridoxine hydrochloride................10.00 mg
Distilled water..........................1.00 L
Filter-sterilize.