【編號】 0057
【名稱】 支原體培養(yǎng)基
【成份】 Hanks 1% casein hydrolyzed liquid 400.0 ml
Serum(horse) 160.0 ml
Ox heart broth 240.0 ml
Yeast extract liquid 16.0 ml
1% Thallium acetate 8.0 ml
Penicillin 200000.0 U
PREPARATION:
(1) Ox heart broth: Immerse 1000g of minced heart into 1600ml water at 80℃ for 10 min.; Chill it to 45℃ and adjust pH8.2-8.5 with Na2CO3
then add pancreatic juice 33ml and chloroform 5-10ml; Digest it at 45℃ for 3 hours
and stir it half hour interval and adjust pH at same time; Adjust pH to 4-4.5 with HCl
boil for 30 min
filtrate with cloth; Adjust the filtrate pH to 7.8-8.0
boil for 30 min
and filtration by paper; Distribute the broth in to flask
autoclave at 8 bl for 20 min.
(2)Yeast extract liquid: Put braking yeast 500g into 1000ml of water
full mix and adjust ph to 5.0; Heat to 80℃ for 30 min; After filtration and distribution
autoclave at 8 bl for 30 min.