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【成份】
0437 脂環(huán)酸芽孢桿菌培養(yǎng)基
Solution A:
CaCl2 ·2H2O 0.25 g
MgSO4·7H2O 0.5 g
(NH4)2 SO4 0.2 g
Yeast extract 2.0 g
Glucose 5.0 g
KH2PO4 3.0 g
Distilled water (for liquid medium) 1.0 L
Distilled water (for solid medium) 0.5 L
Adjust pH to 4.0
Solution B:
Trace element sol. SL-6 (see medium 27) 1.0 ml
Trace element solution SL-6:
ZnSO4 · 7 H2O 0.1 g
MnCl2 · 4 H2O 0.03 g
H3BO3 0.3 g
CoCl2 · 6 H2O 0.2 g
CuCl2 · 2 H2O 0.01 g
NiCl2 · 6 H2O 0.02 g
Na2MoO4 ·2 H2O 0.03 g
Distilled water 1.0 L
Solution C:
Agar 15.0 g
Distilled water 500.0 ml
Sterilize separately. For liquid medium combine solution A (with 1000.0 ml distilled water) and solution B. For solid medium combine solution A (with 500 ml distilled water)
solution B and solution C. For strains of A. cycloheptanicus add 5 g/l of yeast extract instead of 2 g/l.