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平臺編號：bio-116977
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規(guī)格：培養(yǎng)基配方
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用途：/
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
0989
【成份】
0989
KH2PO4 0.3 g
K2HPO4 0.3 g
(NH4)2SO4 0.3 g
NaCl 0.6 g
MgSO4·7H2O 0.13 g
FeSO4·7H2O 2.0 mg
CaCl2·2H2O 8.0 mg
Yeast extract (Difco) 2.0 g
Trypticase peptone (BBL) 2.0 g
Sodium acetate 2.5 g
Sodium formate 2.5 g
Trace minerals (見培養(yǎng)基0893) 10.0 ml
Trace vitamins (見培養(yǎng)基0901) 10.0 ml
NaHCO3 5.0 g
Na2S·9H2O 0.5 g
L-Cysteine·HCl·H2O 0.5 g
Resazurin 1.0 mg
Distilled water 1.0 L
Mix ingredients
except NaHCO3
L-Cysteine·HCl·H2O and Na2S·9H2O
and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20)
and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g.
20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. Separately autoclave cysteine·HCl and Na2S·9H2O as 5% solutions
respectively
under a N2 atmosphere. Prior to inoculation
aseptically and anaerobically add the cysteine·HCl and Na2S·9H2O solutions. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20).
0989
KH2PO4 0.3 g
K2HPO4 0.3 g
(NH4)2SO4 0.3 g
NaCl 0.6 g
MgSO4·7H2O 0.13 g
FeSO4·7H2O 2.0 mg
CaCl2·2H2O 8.0 mg
Yeast extract (Difco) 2.0 g
Trypticase peptone (BBL) 2.0 g
Sodium acetate 2.5 g
Sodium formate 2.5 g
Trace minerals (見培養(yǎng)基0893) 10.0 ml
Trace vitamins (見培養(yǎng)基0901) 10.0 ml
NaHCO3 5.0 g
Na2S·9H2O 0.5 g
L-Cysteine·HCl·H2O 0.5 g
Resazurin 1.0 mg
Distilled water 1.0 L
Mix ingredients
except NaHCO3
L-Cysteine·HCl·H2O and Na2S·9H2O
and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20)
and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g.
20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. Separately autoclave cysteine·HCl and Na2S·9H2O as 5% solutions
respectively
under a N2 atmosphere. Prior to inoculation
aseptically and anaerobically add the cysteine·HCl and Na2S·9H2O solutions. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20).