【成份】
0795
Lab-lemco meat extract (Oxoid) 2.4 g
Proteose peptone No. 3 (Difco) 10.0 g
Yeast extract (Difco) 5.0 g
Na2HPO4 4.0 g
Glucose 1.5 g
Soluble starch 0.5 g
L-Cystine 0. 2 g
Agar 15.0 g
L-Cysteine·HCl·H2O 0.5 g
Horse blood 50.0 g
Distilled water 1.0 L
Add L-cystine to 50 ml of 1N HCl and mix thoroughly. Add remaining components and bring volume to 950 ml. Adjust pH to 7.6 - 7.8. After autoclaving and then cooling to 50C
aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes.