【成份】
0954
(NH4)2SO4 1.3 g
KH2PO4 0.37 g
MgSO4.7H2O 0.25 g
CaCl2 . 2H2O 0.07 g
FeCl3 0.02 g
Glucose 1.0 g
Yeast extract 1.0 g
蒸餾水 1.0 L
Adjust pH to 4.0 with 10 N H2SO4. For solid medium
make up above solution in double strength and adjust pH to 3.5. Make water agar at 40 g/L. Autoclave separately. Cool to about 50C and mix equal volumes of both solutions aseptically. This procedure avoids acid hydrolysis of the agar