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平臺(tái)編號(hào)：bio-117509
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規(guī)格：培養(yǎng)基配方
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用途：/
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
1064
| 【成份】 | 1064 |
| Solution A: | Trypticase peptone (BD BBL) 1.0 g Mineral solution 50.0 ml Trace element solution SL-10 1.0 ml Rumen fluid clarified 50.0 ml Butyric acid 1.7 g Na2SO4 2.8 g Na-resazurin solution (0.1% w F{0XCAB)LKNIT0K@G.gif) ) 0.5 ml蒸餾水 830.0 ml |
| Solution B: | NaHCO3 3.5 g 蒸餾水 50.0 ml |
| Solution C: | Vitamin solution 1.0 ml |
| Solution D: | L-Cysteine-HCl·H2O 0.3 g 蒸餾水 10.0 ml |
| Solution E: | Na2S·9H2O 0.3 g 蒸餾水 10.0 ml Add and dissolve ingredients of solution A |
| Mineral solution： | KH2PO4 10.0 g MgCl2·6 H2O 6.6 g NaCl 8.0 g NH4Cl 8.0 g CaCl2·2 H2O 1.0 g 蒸餾水 1.0 L |
| Trace element solution SL-10： | HCl (25%; 7.7 M) 10.0 ml FeCl2·4 H2O 1.5 g ZnCl2 70.0 mg MnCl2·4 H2O 100.0 mg H3BO3 6.0 mg CoCl2·6 H2O 190.0 mg CuCl2 ·2 H2O 2.0 mg NiCl2·6H2O 24.0 mg Na2MoO4·2H2O 36.0 mg 蒸餾水 990.0 ml First dissolve FeCl2 in the HCl then dilute in water add and dissolve the other salts. Finally make up to 1000.0 ml. |
| Clarified rumen fluid: | Rumen fluid from cow or sheep is filtered through muslin autoclaved at 121°C for 15 min and then centrifuged at 27 000g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C. |
| Seven vitamins solution： | Vitamin B12 100.0 mg p-Aminobenzoic acid 80.0 mg D(+)-Biotin 20.0 mg Nicotinic acid 200.0 mg Calcium pantothenate 100.0 mg Pyridoxine hydrochloride 300.0 mg Thiamine-HCl x· 2 H2O 200.0 mg 蒸餾水 1.0 L |