ATCC Medium: 1245 YPD Medium
Broth Medium
YPD Broth(BD 242820)....................50 g
DI Water............1000 ml
Or for solid medium:
Agar Medium
YPD Agar(BD 242720)..............65 g
DI Water..............1000 ml
Dispense into appropriate vessel and autoclave at 121℃.
*YPD is commercially available from BD.
To make medium from scratch, follow formulation below:
Yeast Extract..............10.0 g
Peptone..............20.0 g
Dextrose................20.0 g
Agar (if needed);................20.0 g
DI Water................1000 ml
Adjust for final pH of 5.6. Autoclave at 121℃.