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ATCC medium: 1754 PETC medium

中國(guó)微生物菌種查詢網(wǎng) / 2016-11-11 14:50:42

 ATCC medium: 1754 PETC medium

NH4Cl ....................................1.0 g

KCl......................................0.1 g

MgSo4 . 7H2O .............................0.2 g

NaCl.....................................0.8 g

KH2PO4 ...................................0.1 g

CaCl2 . 2H2O ............................20.0 mg

Yeast extract............................1.0 g

Trace Elements (see below)..............10.0 ml

Wolfe's Vitamin Solution (see below)....10.0 ml

NaHCO3 ...................................2.0 g

Fructose.................................5.0 g

Reducing Agent (see below)..............10.0 ml

Distilled water........................980.0 ml

Add ingredients through vitamins and adjust to pH 5.5. Add NaHCO3, boil, degas and dispense anaerobically under 100% CO2. Autoclave at 121C for 15 minutes. Add fructose and reducing agents from sterile anaerobic stock solutions prepared under 100% N2. Final pH is 5.9.

Trace Elements:

Nitrilotriacetic acid..............2.0 g

MnSO4 . H2O ........................1.0 g

Fe(SO4)2(NH4)2 . 6H2O ...............0.8 g

CoCl2 . 6H2O .......................0.2 g

ZnSO4 . 7H2O .......................0.2 mg

CuCl2 . 2H2O ......................20.0 mg

NiCl2 . 6H2O ......................20.0 mg

Na2MoO4 . 2H2O.....................20.0 mg

Na2SeO4 ...........................20.0 mg

Na2WO4 ............................20.0 mg

Distilled water....................1.0 L

Add nitrilotriacetic acid to water and adjust to pH 6.0 with KOH. Add remainder of ingredients.

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