ATCC Medium: 2713 Wilkins Chalgren Anaerobic Medium
Wilkins-Chalgren Anaerobe Broth (Oxoid CM0643)……………33 g
DI Water……………………………………………………………..1000 ml
Dispense and autoclave at 121?C
. *This product is commercially available from Oxoid. To make medium from scratch, follow formulation below:
Tryptone……………………………………….10.0 g
Gelatin Peptone………………………………10.0 g
Yeast Extract…………………………………..5.0 g
Glucose………………………………………..1.0 g
Sodium Chloride……………………………...5.0 g
L-Arginine……………………………………..1.0 g
Sodium Pyruvate……………………………..1.0 g
Menadione……………………………………0.0005 g
Haemin………………………………………..0.005 g
DI Water……………………………………….1000 ml
Final pH 7.1 +/- 0.2. Autoclave at 121?.