ATCC Medium: 12 Azotobacter Supplement
K2HPO4……………………………….….1.0 g
MgSO4 x 7H20…………………………...0.2 g
NaCl…………………………………….…0.2 g
FeSO4…………………………………….5.0 mg
Soil Extract (See Below)…………….….100 ml
Mannitol Solution (See Below)…….…...100 ml
Agar (if required)………………………….15.0 g
Tap water………………………….……...800 ml
Adjust base medium to pH 7.6.
Autoclave at 121?C.
Mannitol Solution
Mannitol………………………20 g
DI Water……………………...100 ml
Filter sterilize and add to sterile base medium.
Soil Extract African Violet Soil……………77.0 g
Na2CO3………………………0.2 g
DI water………………………200 ml
Boil for 1 hour. Filter to clarify before use.