ATCC Medium: 7 Actinomyces Broth
Actinomyces Broth (BD 210920)……57 g
DI Water……………………………….1000 ml
Autoclave at 121?C and dispense into appropriate vessel.
*This product is commercially available from BD.
To make medium from scratch, follow formulation below:
BD Actinomyces Composition
Tryptone…………………………..4.0 g
Heart Infusion Broth…………….10 g
Peptone………………………….10 g
NaCl………………………….......5.0 g
K2HPO4…………………….......13.0 g
KH2PO4…………………………2.0 g
Ammonium Sulfate………….....1.0 g
Magnesium Sulfate…………….0.2 g
Calcium Chloride……………….0.01 g
Dextrose…………………………5.0 g
L-CysteineHCl………………….1.0 g
Yeast Extract……………………5.0 g
Soluble Starch……………….....1.0 g
DI Water…………………….......1000 ml
pH 6.9 +/- 0.2 Autoclave at 121?C.