ATCC medium: 1620 CH-1 medium
Yeast extract................5.0 g
Hy-Case SF...................5.0 g
NaCl.......................250.0 g
Tris........................12.0 g
Solution 1 (see below)......50.0 ml
Glycerol....................10.0 g
Distilled water to...........1.0 L
Adjust for a final pH of 7.4.
Autoclave at 121C for 15 minutes.
Solution 1:
MgCl2 . 6H2O ................40.0 g
KCl..........................4.0 g
CaCl2 . 2H2O .................0.4 g
Distilled water..............1.0 L