Yeast extract...............30.0 g
Casamino acids..............20.0 g
Na2HPO4 ......................2.48 g
KH2PO4 .......................0.41 g
MgSO4 . 7H2O ................20.0 mg
MnSO4 . H2O ..................7.5 mg
FeSO4 . 7H2O .................6.4 mg
Citric acid..................6.4 mg
Distilled water............900.0 ml
Adjust for final pH 7.3. Autoclave at 121C for 15 minutes. Cool to room temperature and aseptically add the following filter-sterilized solution:
Sodium pyruvate.............23.2 g
Distilled water............100.0 ml