KH2PO4 .............................4.4 g
Na2HPO4 ............................4.8 g
NH4Cl ..............................1.0 g
MgSO4 . 7H2O .......................0.5 g
Agar..............................20.0 g
Distilled water....................1.0 L
Autoclave.
Filter-sterilize 5.0 ml of Solution A (see below), and 10.0 ml of Solution B (see below), and add to the above.
Solution A:
Ferric ammonium citrate............1.0 g
CaCl2 ..............................0.1 g
Distilled water..................100.0 ml
Solution B:
Glucose...........................10.0 g
Distilled water..................100.0 ml
For autotrophic growth omit Solution B.