HEPES........................1.3 g
MES..........................1.1 g
Yeast extract................1.0 g
Arabinose....................1.0 g
Gluconic acid................1.0 g
KH2PO4 .......................0.22 g
Na2SO4 .......................0.25 g
Agar (for solid medium).....18.0 g
Distilled water..............1.0 L
Add the specified volume of each of the following aqueous stock solutions:
NH4Cl (16.0 g/100 ml) ..............2.0 ml
FeCl3 (0.67 g/100 ml) ..............1.0 ml
CaCl2 (1.5 g/100 ml) ...............1.0 ml
MgSO4 . 7H2O (18.0 g/100 ml) .......1.0 ml
NaMoO4 . 2H2O (1.0 g/100 ml) .......1.0 ml
NiCl2 (1.2 g/100 ml) ...............0.1 ml
After medium is complete, autoclave at 121C for 30 minutes.