ATCC medium: 2139 Chrysiogenes arsenatis medium
Trace Element Solution (see below).............0.1 ml
Distilled water..............................110.0 ml
Boil to remove oxygen.
Autoclave at 121C for 15 minutes. Cool under N2. Aseptically and anaerobically add:
Solution 1 (see below).........................1.0 ml
Solution 2 (see below).........................1.0 ml
5% NaHCO3 Solution (see below) .................1.0 ml
1 M Sodium Acetate Solution (see below)........0.5 ml
1 M Sodium Arsenate Solution (see below).......0.5 ml
Dispense under N2 into Hungate tubes.
Trace Element Solution:
EDTA . 2H2O ..................5.2 g
FeCl2 . 4H2O .................1.5 g
ZnCl2 .......................70.0 mg
MnCl2 . 4H2O ...............100.0 mg
H3BO3 .......................62.0 mg
CoCl2 . 6H2O ...............180.0 mg
CuCl2 . 2H2O ................17.0 mg
NiCl2 . 2H2O ................24.0 ng
Na2MoO4 . 2H2O...............36.0 mg
Distilled water..............1.0 L
Solution 1:
NaCl........................12.0 g
KCl..........................3.0 g
NH4Cl ........................1.5 g
KH2PO4 .......................2.0 g
Na2SO4 .......................0.3 g
Distilled water............110.0 ml
Adjust pH to 7.0. Boil for four minutes to remove oxygen.
Autoclave at 121C for 15 minutes. Keep sealed from air.
Solution 2:
MgCl2 . 6H2O .................4.0 g
CaCl2 . 2H2O .................1.5 g
Distilled water............110.0 ml
Boil for 4 minutes to remove oxygen.
Autoclave at 121C for 15 minutes. Keep sealed from air.
2 5% NaHCO3 Solution:
NaHCO3 .......................5.0 g
Distilled water............110.0 ml
Boil water to remove oxygen. Add sodium bicarbonate. Filter-sterilize.
1 M Sodium Acetate Solution: Sodium acetate...............8.2 g
Distilled water............110.0 ml
Adjust pH to 7.0. Boil for four minutes to remove oxygen. Autoclave at 121C for 15 minutes. Keep sealed from air. 1 M Sodium Arsenate Solution:
Sodium arsenate . 7H2O ......31.2 g
Distilled water............110.0 ml
Adjust pH to 7.0. Boil for four minutes to remove oxygen. Autoclave at 121C for 15 minutes. Keep sealed from air.