Base Medium
Brain Heart Infusion (BD 237500)......................................37.0 g
DI Water……………………………………..........................850.0 ml
Autoclave at 121?C.
.
Cool to 50?C and aseptically add the following:
Additive
Heat inactivated fetal bovine serum………........................100.0 ml
Glucose Solution (see below)………..................................50 ml
Glucose Solution Glucose………………………………………………………..2.0 g
DI Water………………………………………………………..50 ml
Filter sterilize.
Prepare medium anaerobically using 80% N2, 10% CO2, 10% H2.