Dubos Broth Base (BD 238510)...................6.5 g
Agar..........................................15.0 g
Dubos Oleic Albumin Complex (BD 237510)......100.0 ml
Distilled water..............................900.0 ml
Combine all except Dubos Oleic Albumin Complex (DOAC). Heat to boiling to dissolve agar.
Autoclave at 121C for 15 minutes. Aseptically add DOAC.
DOAC: A 0.05% solution of alkalinized oleic acid in a 5% solution of albumin fraction V in normal saline (0.85%).