亚日韩在线中文字幕亚洲,国产在线播放鲁啊鲁视频,张柏芝手扒性器全部图片,亚洲成无码电影在线观看

ATCC medium: 1729 Ruminococcus pasteurii medium

中國(guó)微生物菌種查詢網(wǎng) / 2016-11-25 10:18:25

ATCC medium: 1729 Ruminococcus pasteurii medium

KH2PO4 .........................................0.2 g

NH4Cl ..........................................0.25 g

NaCl...........................................1.0 g

MgCl2 . 6H2O ...................................0.4 g

KCl............................................0.5 g

CaCl2 . 2H2O ...................................0.15 g

Trace Elements Solution SL-7 (see below).......1.0 ml

Resazurin......................................1.0 mg

NaHCO3 .........................................2.5 g

Sodium tartrate................................2.0 g

Na2S . 9H2O ....................................0.36 g

Distilled water................................1.0 L

Adjust medium for final pH 7.2. Boil medium without sodium bicarbonate, trace elements or sodium sulfide under 80% N2, 20% CO2; dispense and tube with the same gas phase. Cool and aseptically add the filtersterilized sodium bicarbonate and trace elements. Aseptically add the sodium sulfide which has been separately autoclaved under N2.

Trace Element Solution SL-7:

Hydrochloric acid, 25%............10.0 ml

FeCl2 . 4H2O .......................1.5 g

CoCl2 . 6H2O .....................190.0 mg

MnCl2 . 4H2O .....................100.0 mg

ZnCl2 .............................70.0 mg

H3BO3 .............................62.0 mg

Na2MoO4 . 2H2O.....................36.0 mg

NiCl2 . 6H2O ......................24.0 mg

CuCl2 . 2H2O ......................17.0 mg

Distilled water....................1.0 L

Dissolve the FeCl2 . 4H2O in the concentrated HCl, then dilute. Use 1.0 ml/L of medium.

上一篇：ATCC medium: 2166 Dubos with oleic albumin

下一篇：ATCC Medium: 1016 Chopped Meat Carbohydrate Medium Complete