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ATCC medium: 731 Luminous medium

中國(guó)微生物菌種查詢(xún)網(wǎng) / 2016-11-28 13:36:29

ATCC medium: 731 Luminous medium

NaCl..............................30.0 g

K2HPO4 .............................3.9 g

KH2PO4 .............................2.1 g

NH4Cl ..............................5.0 g

Yeast extract......................5.0 g

Tryptone...........................5.0 g

1 M Tris buffer, pH 7.5...........50.0 ml

Glycerol...........................3.0 ml

MgSO4 . 7H2O .......................1.0 g

KCl................................0.75 g

CaCO3 ..............................1.0 g

Agar (if needed)..................20.0 g

Distilled water to.................1.0 L

Adjust pH to 7.2.

Autoclave 15 minutes at 121C.

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