Yeast Extract………………1.0 g
Beef Extract………………..1.0 g
Tryptose……………………2.0 g
FeSO4………………...…...0.002 g
Dextrose……………...……10.0 g
Agar………………………..15.0 g
DI Water……………………1000 ml
For broth, eliminate agar and reduce all concentrations to 1/3 of above.
Adjust to pH 7.5 prior to autoclaving for final pH of 7.2. Autoclave at 121?C.