ATCC medium: 210 Modified Starkey's medium C (ATCC medium 207) with 2.5% NaCl
Add 25.0 g of NaCl to ATCC medium 207 (see below).
ATCC medium 207:
Basal medium:
KH2PO4 .......................0.5 g
NH4Cl ........................1.0 g
Na2SO4 .......................1.0 g
CaCl2 . 2H2O .................0.1 g
MgSO4 . 7H2O .................2.0 g
Sodium lactate...............3.5 g
Yeast extract................1.0 g
Tap water....................1.0 L
Adjust pH to 7.6 +/- 0.2. Autoclave 15 minutes at 15 lbs. Cool. Filter off precipitate. Readjust pH to 7.4. Resterilize 20 minutes at 10 lbs. Separately prepare: sterile 2 N NaOH, filter-sterilized 7.5% L-cysteine . HCl, and filter-sterilized 1.0% ferrous ammonium sulfate. Add the above solutions aseptically to 100 ml of the basal medium, just prior to use, in the following proportions:
L-cysteine solution..........1.0 ml
Ferrous ammonium sulfate.....5.0 ml
Adjust pH with sterile NaOH solution to 7.5 +/- 0.3.