Aseptically add 100 ml sterile horse serum to ATCC Medium 646 (see below)
ATCC Medium 646:
Trypticase Peptone (BD 211921)............17.0 g
Phytone Peptone (BD 211906)...............3.0 g
NaCl......................................5.0 g
K2HPO4 ....................................2.5 g
Yeast extract.............................4.0 g
Agar......................................15.0 g
Distilled water...........................1.0 L
Autoclave at 121C for 15 minutes.