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ATCC medium: 510 Acidaminococcus medium (VR)

中國(guó)微生物菌種查詢網(wǎng) / 2016-12-13 08:37:53

ATCC medium: 510 Acidaminococcus medium (VR)

Acid hydrolyzed casein, vitamin- and salt-free......20.0 g

Glucose..............................................5.0 g

VR Salts A (see below)..............................30.0 m

l VR Salts B (see below)...............................4.0 ml

L-Cysteine . HCl.....................................0.35 g

DL-Tryptophan........................................0.1 g

Guanine..............................................0.01 g

Uracil...............................................0.01 g

Hypoxanthine.........................................0.01 g

Pyridoxal............................................1.0 mg

Calcium D-(+)-pantothenate...........................1.0 mg

Thiamine............................................50.0 mcg

Niacin..............................................50.0 mcg

Riboflavin..........................................50.0 mcg

p-Aminobenzoic acid.................................10.0 mcg

Biotin...............................................2.0 mcg

Folic acid...........................................1.0 mcg

Vitamin B12..........................................1.0 mcg

Distilled water......................................1.0 L

Filter-sterilize vitamins as separate stock solution and add aseptically to sterile basal medium. Adjust medium, if necessary, to pH 7.0 with solid K2CO3. Prepare and dispense medium anaerobically using Hungate techniques and 100% N2 gas.

VR Salts A:

Na2HPO4 .....................37.5 g

KH2PO4 ......................12.5 g

H2O ........................500.0 ml

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