ATCC medium: 1685 NYC III
HEPES....................................4.0 g
Proteose Peptone No. 3 (BD 211693)......15.0 g
NaCl.....................................5.0 g
Distilled water........................875.0 ml
Adjust for final pH 7.3. Autoclave at 121C for 15 minutes. Cool to room temperature and add the following filter-sterilized solution:
Glucose..................................5.0 g
Fresh yeast extract (Gibco 360-8180)....25.0 ml
Heat inactivated horse serum...........100.0 ml