ATCC Medium: 2873 Oral Treponenie Isolate (OTI) Medium
Solution A
Polypeptone ……………………………5.0 g
Heart Infusion Broth…………………. ..5.0 g
Yeast Extract (BD 212750)………..…..5.0 g
Pectin…………………………………....0.8 g
Dextrose…………………………………0.8 g
Soluble Starch……………………….....0.8 g
Saccharose……………………………..0.8 g
D-Maltose Monohydrate……………....0.8 g
Sodium Pyvuate……………………... .0.8 g
D-Ribose……………………………… 0.8 g
D-Fructose…………………………… .0.8 g
K2HPO4………………………………….7.0 g
NaCl…………………………………… 5.0 g
MgSO4…………………………………...0.1 g
L-Cysteine hydrochloride……………..0.68 g
Rumen…………………………………500 mL
Agar (broth preparation)………………1.6 g
Agar (for solid media preparation)……15.0 g
Add components and adjust pH to 7.0. Autoclave @121°C.
Cool medium in water bath.
Aseptically add:
Solution B
Thiamine pyrophosphate……………..7.5 mg
Distilled Water…………………………75 mL
Solution C
Yeast Extract #3………………………375 mL
Solution D
Fetal Bovine Serum………………....50 mL
Dispense into sterile vessels Final pH 7.0 +/-0.2