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ATCC medium: 1205 Yeast mannitol agar

中國微生物菌種查詢網(wǎng) / 2017-01-09 13:46:59

ATCC medium: 1205 Yeast mannitol agar

K2HPO4 ...................................0.5 g

MgSO4 . 7H2O .............................0.2 g

NaCl.....................................0.1 g

Mannitol................................10.0 g

Yeast Extract (BD 212750)................0.4 g

Wolfe's Mineral Solution (see below)....10.0 ml

Distilled water to.......................1.0 L

Autoclave at 121C for 15 minutes. Wolfe's Mineral Solution: Available from ATCC as a sterile ready-to-use liquid (Trace Mineral Supplement, catalog no. MD-TMS.)

Nitrilotriacetic acid........1.5 g

MgSO4 . 7H2O .................3.0 g

MnSO4 . H2O ..................0.5 g

NaCl.........................1.0 g

FeSO4 . 7H2O .................0.1 g

CoCl2 . 6H2O .................0.1 g

CaCl2 ........................0.1 g

ZnSO4 . 7H2O .................0.1 g

CuSO4 . 5H2O .................0.01 g

AlK(SO4)2 . 12H2O.............0.01 g

H3BO3 ........................0.01 g

Na2MoO4 . 2H2O................0.01 g

Distilled water..............1.0 L

Add nitrilotriacetic acid to approximately 500 ml of water and adjust to pH 6.5 with KOH to dissolve the compound. Bring volume to 1.0 L with remaining water and add remaining compounds one at a time.

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