ATCC medium: 2069 Minimal Medium for Streptococcus bovis
K2HPO4 ...........................292.0 mg
KH2PO4 ...........................292.0 mg
(NH4)2SO4.........................480.0 mg
NaCl.............................480.0 mg
MgSO4 . 7H2O .....................100.0 mg
CaCl2 . 2H2O ......................64.0 mg
Hemin..............................1.0 mg
Wolfe's Vitamin Solution..........10.0 ml
Glucose............................4.0 g
L-Cysteine . HCl...................0.6 g
Distilled water....................1.0 L
Add components through vitamins; boil and cool under 100% N2. Add
glucose and cysteine. Adjust medium for final pH 6.7. Tube under 100% N2
using Hungate techniques. Autoclave at 121C for 15 minutes.
Wolfe's Vitamin Solution:
Available from ATCC as a sterile ready-to-use liquid (Vitamin
Supplement, catalog no. MD-VS).
Biotin.......................2.0 mg
Folic acid...................2.0 mg
Pyridoxine hydrochloride....10.0 mg
Thiamine . HCl...............5.0 mg
Riboflavin...................5.0 mg
Nicotinic acid...............5.0 mg
Calcium D-(+)-pantothenate...5.0 mg
Vitamin B12..................0.1 mg
p-Aminobenzoic acid..........5.0 mg
Thioctic acid................5.0 mg
Distilled water..............1.0 L