ATCC Medium: 1256 Sulfolobus Medium
(NH4)2SO4..................................................1.3 g
KH2PO4.......................................................0.28 g
MgSO4 x 7H2O……………..........................0.25 g
CaCl2 x 2H2O .............................................0.07 g
FeCl3 x 6H2O……………….........................0.02 g
MnCl2 x 4H2O………………........................1.8 mg
Na2B4O7 x 10H2O……………....................4.5 mg
ZnSO4 x 7H2O ...........................................0.22 mg
CuCl2 x 2H2O………………........................0.05 mg
Na2MoO4 x 2H2O.......................................0.03 mg
VOSO4 x 2H2O…………….........................0.03 mg
CoSO4…………………................................0.01 mg
Yeast Extract…………………………...........1.0 g
DI Water………………………………………1000 ml
Adjust pH to 2.0 with 10 N H2SO4 (at room temperature). Filter-sterilize and
dispense into appropriate vessel.