ATCC medium: 73 YGC medium
Yeast extract...............10.0 g
Glucose.....................20.0 g
CaCO3 .......................20.0 g
Agar........................20.0 g
Distilled water..............1.0 L
Autoclave at 10 lbs. for 30 minutes. Place tubes in 48C water bath.
Resuspend calcium carbonate by vibrating individual tubes and slant.