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ATCC; Medium 1849: Harpo's HTYEM (marine) medium

中國(guó)微生物菌種查詢網(wǎng) / 2016-11-09 08:06:05

ATCC medium: 1849 Harpo's HTYEM (marine) medium

Trypticase Peptone (BD 211921)......5.0 g

Yeast extract.......................2.0 g

HEPES...............................4.0 g

Artificial Seawater (see below).....1.0 L

Adjust for final pH 7.5 with NaOH. Autoclave at 121C for 15 minutes. Artificial Seawater:

NaCl................................27.5 g

MgCl2 . 6H2O ........................5.38 g

MgSO4 . 7H2O ........................6.78 g

KCl.................................0.72 g

NaHCO3 ..............................0.2 g

CaCl2 . 2H2O ........................1.4 g

Distilled water.....................1.0 L

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