ATCC幽門螺桿菌（Helicobacter pylori，Hp）是一種專性微需氧菌。它的穩(wěn)定生長需要依靠在生長的微環(huán)境中含2-8%的O2。因此，它在大氣中和絕對厭氧條件下均不能生長。從臨床標(biāo)本中分離野生株都必需補(bǔ)充適量的CO2。而且它生長緩慢，通常需要3-5d，甚至更長時間，才能形成針尖狀小菌落。為了避免快速生長的兼性厭O2菌和霉菌等的過度生長，常需加入由萬古霉素、TMP、兩性霉素、多粘菌素等組合的抑菌劑。 幽門螺桿菌在液體培養(yǎng)基中生長更加困難。這可能與在液體里面更難保證菌體周圍穩(wěn)定的微含O2量環(huán)境有關(guān)。 在血瓊脂中，幽門螺桿菌不能在含有5%膽汁的情況下生長。將細(xì)菌暴露于含有5%膽汁的液體培養(yǎng)基中30min，有25%的幽門螺桿菌被殺死。這說明幽門螺桿菌通過十二指腸時尚有生存的可能性。
菌種名稱：幽門螺桿菌
Helicobacter pylori (Marshall et al.) Goodwin et al. 拉丁名
(ATCC? 49503?) 統(tǒng)一編號
Strain Designations ATCC菌種別名 60190
Application 用途 Enteric Research；
Isolation 分離基物 Human gastric antrum
Biosafety Level 生物安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Storage Conditions 保藏條件
Frozen 冷凍物: -80℃ or colder
Freeze-Dried 凍干物: 2℃ to 8℃
Live Culture 活物: See Propagation Section
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Preceptrol? no
Type Strain 模式菌株 no
Comments 注釋 Cytotoxin activity and serologic response
Medium 培養(yǎng)基 ATCC? Medium 2856: Brucella Agar/Broth w/ 10% FBS
ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions 生長條件 Temperature 培養(yǎng)溫度: 37℃
Atmosphere 需氧情況: Microaerophilic
Name of Depositor 寄存人 TL Cover U.S. Patent Number 5,618,840
Chain of Custody 來源國家 ATCC <-- TL Cover <-- R. Leunk <-- A. Pearson and P. Hawtin
References 參考文獻(xiàn) Cover TL, et al. Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infect. Immun. 58: 603-610, 1990. PubMed: 2307514
Cao P, Cover TL. High-level genetic diversity in the vapD chromosomal region of Helicobacter pylori. J. Bacteriol. 179: 2852-2856, 1997. PubMed: 9139899
Melchers K, et al. Cloning and membrane topology of a P type ATPase from Helicobacter pylori. J. Biol. Chem. 271: 446-457, 1996. PubMed: 8550601
Dubois A, et al. Transient and persistent experimental infection of nonhuman primates with Helicobacter pylori: implications for human disease. Infect. Immun. 64: 2885-2891, 1996. PubMed: 8757808
Cross References Nucleotide (GenBank) : Y08762 Helicobacter pylori flhA gene and unknown ORF.
Nucleotide (GenBank) : AC002058 Helicobacter pylori, *** SEQUENCING IN PROGRESS ***, in ordered pieces.
Nucleotide (GenBank) : U05676 Helicobacter pylori 60190 cysteinyl-tRNA synthetase homolog gene, partial cds, and vacuolating cytotoxin (vacA) gene, complete cds.