波特氏桿菌：ATCC菌種
Bordetella petrii von Wintzingerode 拉丁名
(ATCC? BAA-461?) 統(tǒng)一編號(hào)
Strain Designations 別名 CCUG 43448 [CIP 107267, DSM 12804, Se-1111R]
Application 用途 Reduces selenic acid selenate
Isolation 分離基物 Anaerobic, dechlorinating bioreactor culture enriched from river sediment, 1998
Biosafety Level 生物安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保存方法 Frozen 冷凍物 : -80°C or colder
Freeze-Dried 凍干粉 : 2°C to 8°C
Live Culture 活物 : See Propagation Section
Preceptrol? no
Type Strain 模式菌株 yes
Medium 培養(yǎng)基 ATCC? Medium 3: Nutrient agar or nutrient broth
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 37°C
Atmosphere 需氧情況 : Aerobic
Name of Depositor 寄存者 CCUG
Special Collection NSF - Bacteriology
Chain of Custody 來源國家 ATCC <-- CCUG <-- CCUG <-- F. von Winzingerode
References 參考文獻(xiàn) von Wintzingerode F, et al. Bordetella petrii sp. nov., isolated from an anaerobic bioreactor, and emended description of the genus Bordetella. Int. J. Syst. Evol. Microbiol. 51: 1257-1265, 2001. PubMed: 11491321
Cross References
Nucleotide (GenBank) : AJ242553 Bordetella petri risA gene, strain SE1111R.
Nucleotide (GenBank) : AJ249861 Bordetella petrii 16S rRNA gene, strain DSM 12804.
Nucleotide (GenBank) : AJ242599 Bordetella petri ompA gene for putative outer membrane protein.
ATCC?培養(yǎng)基3：營養(yǎng)瓊脂或營養(yǎng)肉湯
生長條件溫度：37°C氣氛：有氧
傳播程序
1.根據(jù)隨附的說明打開樣品瓶。
2.從單管＃3肉湯（5至6 mL）中，用巴斯德或1.0 mL移液管取出約0.5至1.0 mL，并用于再水化整個(gè)顆粒。
3.將再水化的顆粒無菌轉(zhuǎn)移回肉湯管中。 好好混合。
4.使用幾滴該懸浮液接種第二管肉湯，斜面和/或平板。
5.將管和板在37℃下孵育48小時(shí)
ATCC? Medium 3: Nutrient agar or nutrient broth
Growth Conditions Temperature: 37°C Atmosphere: Aerobic
Propagation Procedure
1.Open vial according to enclosed instructions.
2.From a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette and use to rehydrate the entire pellet.
3.Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4.Use several drops of this suspension to inoculate a second tube of broth, a slant and/or a plate.
5.Incubate the tubes and plates at 37°C for 48 hours
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